Library Synthesis: Building Block Selection, Handling, and Tracking

Katelyn J Billings; Melissa C Grenier-Davies

doi:10.1007/978-1-0716-2545-3_1

Library Synthesis: Building Block Selection, Handling, and Tracking

Methods Mol Biol. 2022:2541:1-11. doi: 10.1007/978-1-0716-2545-3_1.

Authors

Katelyn J Billings¹, Melissa C Grenier-Davies²

Affiliations

¹ Relay Therapeutics, Cambridge, MA, USA. kbillings@relaytx.com.
² Encoded Library Technologies/NCE Molecular Discovery, R & D Medicinal Science and Technology, GlaxoSmithKline, Cambridge, MA, USA.

PMID: 36083536
DOI: 10.1007/978-1-0716-2545-3_1

Abstract

Careful selection and manipulation of small molecule building blocks is crucial to the success of a DNA-encoded library. Building block selection impacts the quality of the hits arising out of a selection assay, while proper sample handling and tracking ensure follow-up synthetic work is done with the appropriate synthetic map in mind. In this chapter, possible strategies for building block selection are outlined, as well as best practices for handling and tracking samples to be used for validation and library synthesis.

Keywords: 2D barcoded tubes; Building blocks; DNA-encoded library; Diversity selection; Library optimization; Property filters; Reactivity; Small molecules.

MeSH terms

Gene Library*