We successfully synthesized glyco-micromotors in situ directed by multifunctional glycopolymers, which were obtained by copolymerizing 2-methacrylamido glucopyranose (MAG) and 2-(diethylamino)ethyl methacrylate (DEAEMA) monomers. The fabricated glyco-micromotors present abilities in loading and guiding bacteria with different individual and group motions.