Mouse Models for Cytomegalovirus Infections in Newborns and Adults

Ilija Brizić; Berislav Lisnić; Fran Krstanović; Wolfram Brune; Hartmut Hengel; Stipan Jonjić

doi:10.1002/cpz1.537

Mouse Models for Cytomegalovirus Infections in Newborns and Adults

Curr Protoc. 2022 Sep;2(9):e537. doi: 10.1002/cpz1.537.

Authors

Ilija Brizić¹, Berislav Lisnić¹, Fran Krstanović¹, Wolfram Brune², Hartmut Hengel³, Stipan Jonjić¹

Affiliations

¹ Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
² Leibniz Institute for Virology, Hamburg, Germany.
³ Institute of Virology, Medical Center-University of Freiburg, and Faculty of Medicine, University of Freiburg, Freiburg, Germany.

PMID: 36083111
DOI: 10.1002/cpz1.537

Abstract

This article describes procedures for infecting adult mice with murine cytomegalovirus (MCMV) and for infecting newborn mice to model congenital CMV infection. Methods are included for propagating MCMV in cell cultures and preparing a more virulent form of MCMV from the salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, methods are described for preparing the murine embryonic fibroblasts used for propagating MCMV, and for the plaque assay. © 2022 Wiley Periodicals LLC.

Keywords: MCMV; cytomegalovirus infections; disease models; fibroblasts; herpesviridae infections; mice; mouse cytomegalovirus; salivary gland; viral load; viral plaque assay; virology; virus replication.

MeSH terms

Animals
Cytomegalovirus Infections*
Disease Models, Animal
Mice
Muromegalovirus*
Salivary Glands

Abstract

MeSH terms

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