Optimization of the CYP inhibition assay using LC-MS/MS

Muhammad Asyraf Abduraman; Nor Hidayah Mustafa; Nik Soriani Yaacob; Azimah Amanah; Mei Lan Tan

doi:10.1016/j.mex.2022.101827

Optimization of the CYP inhibition assay using LC-MS/MS

MethodsX. 2022 Aug 23:9:101827. doi: 10.1016/j.mex.2022.101827. eCollection 2022.

Authors

Muhammad Asyraf Abduraman¹, Nor Hidayah Mustafa², Nik Soriani Yaacob³, Azimah Amanah⁴, Mei Lan Tan¹

Affiliations

¹ School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia.
² Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia.
³ School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia.
⁴ Malaysian Institute of Pharmaceuticals & Nutraceuticals (IPharm), National Institutes of Biotechnology Malaysia (NIBM), Pulau Pinang 11700, Malaysia.

Abstract

The data presented in this article are related to the research article entitled "Cytochrome P450 inhibition activities of non-standardized botanical products" [1], in which the possible CYP inhibitory properties of botanical products were investigated. This article describes the optimization and bioanalytical method validation of the CYP (Cytochrome P450 inhibition assay) inhibition assays, namely, phenacetin O-deethylase assay, testosterone 6β-hydroxylase assay, felodipine dehydrogenase assay and midazolam 1'-hydroxylase assay using LC-MS/MS.