Aptamers-functionalized nanoscale MOFs for saxitoxin and tetrodotoxin sensing in sea foods through FRET

Xilin Dou; Shihan Xu; Yifei Jiang; Zhaoyang Ding; Jing Xie

doi:10.1016/j.saa.2022.121827

Aptamers-functionalized nanoscale MOFs for saxitoxin and tetrodotoxin sensing in sea foods through FRET

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jan 5:284:121827. doi: 10.1016/j.saa.2022.121827. Epub 2022 Sep 5.

Authors

Xilin Dou¹, Shihan Xu², Yifei Jiang², Zhaoyang Ding³, Jing Xie⁴

Affiliations

¹ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
² Department of Chemistry, University of Washington, Seattle, WA 98195, USA.
³ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. Electronic address: zyding@shou.edu.cn.
⁴ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China. Electronic address: jxie@shou.edu.cn.

PMID: 36081191
DOI: 10.1016/j.saa.2022.121827

Abstract

Saxitoxin (STX) and tetrodotoxin (TTX) are widely distributed and extremely harmful marine toxins, it is certainly worth to spend effort to develop facile methods to detect them in sea food for human safety. In this work, two nano-sensors were developed by combining with two zirconium fluorescence Nanoscale metal-organic frameworks (NMOFs) with two emissions and TAMRA-labelled aptamers for STX and TTX sensing, respectively. The recognition of STX and TTX by these nano-sensors could change the structure of aptamer, which caused the blue or green emissions from NMOFs (energy donor) decreased while red emission from TAMRA-labelled aptamers (energy acceptor) increased owing to fluorescence resonance energy transfer (FRET) effect. Based on this strategy, NMOFs-Aptasensor 1 and NMOFs-Aptasensor 2 were developed for the ratiometric detection, with detection limits of 1.17 nM and 3.07 nM for STX and TTX, respectively. Moreover, NMOFs-Aptasensors displayed significant stability, pH-independence, selectivity and NMOFs-Aptasensors were successfully applied in shellfish sample for toxin sensing.

Keywords: Aptamer; FRET; Fluorescence sensing; MOF; Saxitoxin; Tetrodotoxin.

MeSH terms

Aptamers, Nucleotide* / chemistry
Fluorescence Resonance Energy Transfer
Humans
Marine Toxins
Metal-Organic Frameworks*
Saxitoxin / chemistry
Seafood / analysis
Tetrodotoxin
Zirconium

Substances

Aptamers, Nucleotide
Marine Toxins
Metal-Organic Frameworks
Saxitoxin
Tetrodotoxin
Zirconium