Revealing the species-specific genotype of the edible bird's nest-producing swiftlet, Aerodramus fuciphagus and the proteome of edible bird's nest

Hang-Kin Kong; Zoe Chan; Sau-Woon Yan; Pak-Yeung Lo; Wing-Tak Wong; Ka-Hing Wong; Chun-Lap Lo

doi:10.1016/j.foodres.2022.111670

Revealing the species-specific genotype of the edible bird's nest-producing swiftlet, Aerodramus fuciphagus and the proteome of edible bird's nest

Food Res Int. 2022 Oct:160:111670. doi: 10.1016/j.foodres.2022.111670. Epub 2022 Jul 12.

Authors

Hang-Kin Kong¹, Zoe Chan², Sau-Woon Yan², Pak-Yeung Lo², Wing-Tak Wong², Ka-Hing Wong³, Chun-Lap Lo⁴

Affiliations

¹ Department of Applied Biology and Chemical Technology, Faculty of Applied Science and Textiles, The Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, China; Research Institute for Future Food, The Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, China. Electronic address: hang-kin.kong@polyu.edu.hk.
² Department of Applied Biology and Chemical Technology, Faculty of Applied Science and Textiles, The Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, China.
³ Department of Applied Biology and Chemical Technology, Faculty of Applied Science and Textiles, The Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, China; Research Institute for Future Food, The Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, China. Electronic address: kahing.wong@polyu.edu.hk.
⁴ Department of Applied Biology and Chemical Technology, Faculty of Applied Science and Textiles, The Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, China; Research Institute for Future Food, The Hong Kong Polytechnic University, Hung Hom, Hong Kong SAR, China. Electronic address: samuel.chun-lap.lo@polyu.edu.hk.

PMID: 36076383
DOI: 10.1016/j.foodres.2022.111670

Abstract

Only a few species of swiftlets in the Aerodramus and Collocalia genera can produce edible bird's nests (EBN). These saliva-cemented nests have been consumed as delicacies for centuries in Asia. Many researches have reported the aqueous extract of EBN has epidermal growth factor-like (EGF-like) activity. However, no standalone EGF has been identified in EBN. Moreover, proteome of EBN remained unclear due to lack of genomic data base of an EBN-producing swiftlet to support proteomic analysis of EBN. To address this, the first genome of the EBN-producing swiftlet, Aerodramus fuciphagus, was constructed. Orthology comparison of A. fuciphagus with 10 other avian species were conducted. The results revealed that the number of predicted paralogous coiled-coil domain-containing protein 63 (CCDC63) coding sequences (CDSs) in A. fuciphagus was found to be significantly expanded in comparison to Gallus gallus. There were 3 paralogous CCDC63 genes in the genome of A.fuciphagus. The CDSs predicted from the genome of A. fuciphagus were used to construct a database for proteomic analysis of EBN. In total, 398 proteins have been identified in EBN. The proteome of EBN was significant enriched with extracellular proteins as well as proteins related to extracellular matrix (ECM) organization and immune response. A few proteins with Ca²⁺-binding EGF-like domains were found in the proteome of EBN, like fibrillin-1, protocadherin fat 4 and coagulation factor X. No standalone EGF protein was identified. This indicated that the proteins with EGF-like domains might be responsible for the EGF-like activity of EBN. In addition, acidic mammalian chitinase and lysyl oxidase in EBN were found to be active when extracting with distilled water at room temperature. The current study has not just revealed the species-specific genotype of the EBN-producing swiftlet, A. fuciphagus, but also revealed the proteome of EBN. This established an important foundation for subsequently studies on efficacies of EBN.

Keywords: Aerodramus fuciphagus; CCDC63; Edible bird’s nest; Epidermal growth factor; Genome; Proteome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Birds / genetics
Birds / metabolism
Epidermal Growth Factor / genetics
Epidermal Growth Factor / metabolism
Genotype
Mammals
Proteome* / genetics
Proteome* / metabolism
Proteomics*

Substances

Proteome
Epidermal Growth Factor