High-throughput scNMT protocol for multiomics profiling of single cells from mouse brain and pancreatic organoids

STAR Protoc. 2022 Aug 29;3(3):101555. doi: 10.1016/j.xpro.2022.101555. eCollection 2022 Sep 16.

Abstract

Single-cell nucleosome, methylome, and transcriptome (scNMT) sequencing is a recently developed method that allows multiomics profiling of single cells. In this scNMT protocol, we describe profiling of cells from mouse brain and pancreatic organoids, using liquid handling platforms to increase throughput from 96-well to 384-well plate format. Our approach miniaturizes reaction volumes and incorporates the latest Smart-seq3 protocol to obtain higher numbers of detected genes and genomic DNA (gDNA) CpGs per cell. We outline normalization steps to optimally distribute per-cell sequencing depth. For complete details on the use and execution of this protocol, please refer to Clark (2019), Clark et al. (2018), and Clark et al., 2018, Hagemann-Jensen et al., 2020a, Hagemann-Jensen et al., 2020b.

Keywords: Bioinformatics; Cell Biology; Genomics; Molecular Biology; RNAseq; Sequence analysis; Sequencing; Single Cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain
  • Epigenome*
  • Mice
  • Nucleosomes*
  • Organoids
  • Transcriptome

Substances

  • Nucleosomes