Biosynthesis of silver nanoparticles with antibacterial, antioxidant, anti-inflammatory properties and their burn wound healing efficacy

Bum-Erdene Bold; Enerelt Urnukhsaikhan; Tsogbadrakh Mishig-Ochir

doi:10.3389/fchem.2022.972534

Biosynthesis of silver nanoparticles with antibacterial, antioxidant, anti-inflammatory properties and their burn wound healing efficacy

Front Chem. 2022 Aug 22:10:972534. doi: 10.3389/fchem.2022.972534. eCollection 2022.

Authors

Bum-Erdene Bold^{1

2}, Enerelt Urnukhsaikhan^{1

2}, Tsogbadrakh Mishig-Ochir^{1

2}

Affiliations

¹ Laboratory of Molecular and Cellular Biophysics, Department of Biology, National University of Mongolia, Ulaanbaatar, Mongolia.
² Graduate School of National University of Mongolia, Ulaanbaatar, Mongolia.

Abstract

The current study aims to develop a novel burn wound ointment consisting of sheep's tail ointment loaded with AgNP. The AgNP in the ointment serves as an antibacterial, antioxidant and anti-inflammatory agent. The AgNP was developed via the biological method with the assistance of the medicinal plant Rhodiola rosea. The characterization of AgNP was assessed using UV-Vis spectroscopy, FTIR, Zeta Potential, XRD, PCCS, SEM, and EDX techniques. The formation of AgNP was confirmed by UV-Vis spectrum at the absorbance of ∼430 nm, and the biomolecules responsible for reducing and capping the AgNP were characterized by FTIR analysis. The stability of AgNP was determined with Zeta potential, which revealed a highly stable colloidal solution with a surface charge of -68.38 ± 3.4 mV. The synthesized AgNP had a face-centered cubic structure with a crystallite size of 23 nm and average grain size of 67.5 nm. The SEM image showed a fairly monodisperse 20 nm-sized spherical-shaped AgNP. The synthesized AgNP contained high purity of the silver, and a low concentration of AgNP inhibited both Gram-positive and Gram-negative bacteria. Moreover, the scavenging activity of AgNP was investigated using DPPH and H₂O₂ scavenging assay, and the results revealed a dose-dependent antioxidant activity with the highest activity at a concentration of 450 μg/ml. Finally, the burn wound healing effect was evaluated by applying the AgNP-loaded ointment to the wound site of BALB/c mice. The in-vivo studies confirmed that AgNP-loaded ointment reduced the wound size, decreased the epidermis layer, and lowered mast cell migration compared to untreated burn wounds. And the synthesized AgNP regulated both pro-inflammatory and anti-inflammatory gene expression, thereby promoting burn wound closure on BALB/c mice. The developed AgNP-loaded ointment has the potential to be applied in the biomedical field.

Keywords: anti-inflammatory activity; anti-oxidant activity; antibacterial activity; biosynthesis; burn injury; silver nanoparticle; wound ointment.