Furostane Saponins from the Seeds of Allium ramosum and Their Lipid Accumulation Inhibitory Activity

Abstract

Three new furostane saponins, ramofurosides A-C (1-3), and two known saponins, fistulosaponin B (4) and (25R)-26-O-β-D-glucopyranosyl-1β,3β,26-trihydroxyfurosta-5,20(22)-diene-1-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside (5) were isolated from the methanol extract of Allium ramosum seeds. Their structures were identified based on spectroscopic evidence and comparison with those reported in the literature. All compounds were evaluated for reduction of lipid accumulation in HepG2 cell lines. As a result, compounds 1 and 3 showed a significant reduction in total lipid content by 27.93±3.05 and 27.54±1.68 %, respectively, at a concentration of 100 μM.

Keywords: Allium ramosum; Amaryllidaceae; furostane saponin; lipid accumulation; ramofuroside.