Simultaneous affinity maturation and developability enhancement using natural liability-free CDRs

Andre A R Teixeira; Sara D'Angelo; M Frank Erasmus; Camila Leal-Lopes; Fortunato Ferrara; Laura P Spector; Leslie Naranjo; Esteban Molina; Tamara Max; Ashley DeAguero; Katherine Perea; Shaun Stewart; Rebecca A Buonpane; Horacio G Nastri; Andrew R M Bradbury

doi:10.1080/19420862.2022.2115200

Simultaneous affinity maturation and developability enhancement using natural liability-free CDRs

MAbs. 2022 Jan-Dec;14(1):2115200. doi: 10.1080/19420862.2022.2115200.

Authors

Affiliations

¹ New Mexico Consortium, Los Alamos, NM, USA.
² Specifica Inc, Santa Fe, NM, USA.
³ Incyte Research Institute, Wilmington, DE, USA.

Abstract

CDR: complementarity determining region; FACS: fluorescence-activated cell sorting; k_a: association rate; k_d: dissociation rate; K_D: dissociation constant; scFv: single-chain variable fragment; SPR: surface plasmon resonance.

Keywords: Affinity maturation; CDR shuffling; developability; liability; library construction; off-rate; scFv.

MeSH terms

Antibody Affinity
Complementarity Determining Regions
Single-Chain Antibodies*
Surface Plasmon Resonance

Substances

Complementarity Determining Regions
Single-Chain Antibodies

Grants and funding

The author(s) reported there is no funding associated with the work featured in this article.