Multiplex short tandem repeat profiling of immortalized hepatic stellate cell line Col-GFP HSC

Steffen K Meurer; David A Brenner; Ralf Weiskirchen

doi:10.1371/journal.pone.0274219

Multiplex short tandem repeat profiling of immortalized hepatic stellate cell line Col-GFP HSC

PLoS One. 2022 Sep 6;17(9):e0274219. doi: 10.1371/journal.pone.0274219. eCollection 2022.

Authors

Steffen K Meurer¹, David A Brenner², Ralf Weiskirchen¹

Affiliations

¹ Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, Aachen, Germany.
² Department of Medicine, University of California San Diego, La Jolla, CA, United States of America.

Abstract

Misidentification, cross-contamination and genetic drift of continuous animal cell lines are persistent problems in biomedical research, leading to erroneous results and inconsistent or invalidated studies. The establishment of immortalized hepatic stellate cell line Col-GFP HSC was reported in PLoS One in the year 2013. In the present study a multi loci short tandem repeat signature for this cell line was established that allows for unique cell line authentication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Hepatic Stellate Cells*
Kupffer Cells
Microsatellite Repeats*

Grants and funding

The laboratory of R.W. is supported by the German Research Foundation (grants WE2554/13-1, WE2554/15-1, and WE2554/17-1). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.