Mechanism of microbiologically induced calcite precipitation for cadmium mineralization

Abstract

Microbiologically induced calcite precipitation (MICP) technology shows potential for remediating heavy metal pollution; however, the underlying mechanism of heavy metal mineralization is not well-understood, limiting the application of this technology. In this study, we targeted Cd contamination (using 15:1, 25:1, and 50:1 Ca²⁺/Cd²⁺ molar ratios) and showed that the ureolytic bacteria Sporosarcina ureilytica ML-2 removed >99.7 % Cd²⁺ with a maximum fixation capacity of 75.61 mg-Cd/g-CaCO₃ and maximum precipitation production capacity of 135.99 mg-CaCO₃/mg-cells. Quantitative PCR analysis showed that Cd²⁺ inhibited the expression of urease genes (ureC, ureE, ureF, and ureG) by 70 % in the ML-2 strain. Additionally, the pseudo-first-order kinetics model (R² = 0.9886), intraparticle diffusion model (R² = 0.9972), and Temkin isotherm model (R² = 0.9828) described the immobilization process of Cd²⁺ by bio calcite in MICP-Cd system. The three Cd²⁺ mineralization products generated by MICP were attributed to surface precipitation (Cd²⁺ → Cd(OH)₂), direct binding with the CO₃^2-/substitution calcium site of calcite (Cd²⁺ → CdCO₃, otavite), and calcite lattice vacancy anchors (Cd²⁺ → (Ca_xCd_1-x)CO₃). Our findings improve the understanding of the mechanisms by which MICP can achieve in situ stabilization of heavy metals.

Keywords: Adsorption model; Cd; MICP; Mineralized species; Molar ratio; Urease gene.