Zebrafish Danio rerio myotomal muscle structure and growth from a spatial transcriptomics perspective

Guanting Liu; Takumi Ito; Yusuke Kijima; Kazutoshi Yoshitake; Shuichi Asakawa; Shugo Watabe; Shigeharu Kinoshita

doi:10.1016/j.ygeno.2022.110477

Zebrafish Danio rerio myotomal muscle structure and growth from a spatial transcriptomics perspective

Genomics. 2022 Sep;114(5):110477. doi: 10.1016/j.ygeno.2022.110477. Epub 2022 Sep 1.

Authors

Guanting Liu¹, Takumi Ito¹, Yusuke Kijima², Kazutoshi Yoshitake¹, Shuichi Asakawa¹, Shugo Watabe³, Shigeharu Kinoshita⁴

Affiliations

¹ Department of Aquatic Bioscience, Graduate School of Agriculture and Life Science, The University of Tokyo, Yayoi 1-1-1, Bunkyo, Tokyo 113-8657, Japan.
² Department of Aquatic Bioscience, Graduate School of Agriculture and Life Science, The University of Tokyo, Yayoi 1-1-1, Bunkyo, Tokyo 113-8657, Japan; School of Biomedical Engineering, Faculty of Applied Science and Faculty of Medicine, The University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
³ Kitasato University School of Marine Biosciences, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan.
⁴ Department of Aquatic Bioscience, Graduate School of Agriculture and Life Science, The University of Tokyo, Yayoi 1-1-1, Bunkyo, Tokyo 113-8657, Japan. Electronic address: kinoshita-s@g.ecc.u-tokyo.ac.jp.

PMID: 36058475
DOI: 10.1016/j.ygeno.2022.110477

Abstract

Fish exhibit different muscle structures and growth characteristics compared with mammals. We used a spatial transcriptomics approach and examined myotomal muscle sections from zebrafish. Adult muscles were divided into eight regions according to spatial gene expression characteristics. Slow muscle was located in the wedge-shaped region near the lateral line and at the base of the dorsal fin, intermediate muscle was located in a ribbon-shaped region adjacent to slow muscle, and fast muscle was located in the deep region of the trunk, surrounded by intermediate muscle; the interior of fast muscle was further divided into 6 parts by their transcriptomic features. Combined analysis of adult and larval data revealed that adult muscles contain specific regions similar to larval muscles. These regions showed active myogenesis and a high expression of genes associated with muscle hyperplasia. This is the first study to apply spatial transcriptomics to fish myotomal muscle structure and growth.

Keywords: Muscle growth; Muscle structure; Spatial transcriptomics; Zebrafish.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Larva
Mammals
Muscle Development / genetics
Muscles
Transcriptome*
Zebrafish* / genetics