Quantitative detection of mycobacterial mannophosphoinositides in tuberculosis patients by real-time immuno-PCR assay

Promod K Mehta; Suman Sharma; Neeru Mehta; Bhawna Dahiya; Pamela Singh; Kavita Prashar; Abhishek Sheoran; Mandira Varma-Basil; Gopal K Khuller

doi:10.1016/j.mimet.2022.106563

Quantitative detection of mycobacterial mannophosphoinositides in tuberculosis patients by real-time immuno-PCR assay

J Microbiol Methods. 2022 Oct:201:106563. doi: 10.1016/j.mimet.2022.106563. Epub 2022 Aug 31.

Authors

Promod K Mehta¹, Suman Sharma², Neeru Mehta³, Bhawna Dahiya⁴, Pamela Singh⁵, Kavita Prashar⁶, Abhishek Sheoran⁷, Mandira Varma-Basil⁸, Gopal K Khuller⁹

Affiliations

¹ Centre for Biotechnology, Maharshi Dayanand University, Rohtak 124001, India. Electronic address: pkmehta3@hotmail.com.
² Centre for Biotechnology, Maharshi Dayanand University, Rohtak 124001, India; Dept. of Microbiology, Pt. B. D. Sharma University of Health Sciences, Rohtak 124001, India.
³ Department of Medical Electronics, Ambedkar Delhi Skill & Entrepreneurship University, Shakarpur, Delhi 110092, India.
⁴ Centre for Biotechnology, Maharshi Dayanand University, Rohtak 124001, India.
⁵ Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal 131039, India.
⁶ Badrinarayan Barwale Mahavidyalaya, Dr Babasaheb Ambedkar Marathwada University, Jalna, Maharashtra 431213, India.
⁷ Department of Statistics, Ramanujan College, University of Delhi, New Delhi 110019, India.
⁸ Microbiology Department, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi-110007, India.
⁹ Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India.

PMID: 36057405
DOI: 10.1016/j.mimet.2022.106563

Abstract

A real-time immuno-PCR assay was deliberated to detect mycobacterial mannophosphoinositides (PIMs). A dynamic range of PIMs (0.9 pg/mL-10 ng/mL) was detected in TB patients, wherein 88.2% and 81.1% sensitivities were obtained in pulmonary TB and extrapulmonary TB respectively, with 96-96.4% specificity. This assay may translate into a diagnostic kit.

Keywords: Diagnosis; ELISA; Mtb; PIMs; Real-time immuno-PCR; TB.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Mycobacterium tuberculosis* / genetics
Phosphatidylinositols
Sensitivity and Specificity
Tuberculosis* / diagnosis
Tuberculosis* / microbiology
Tuberculosis, Pulmonary* / diagnosis

Substances

Phosphatidylinositols
mannophosphoinositide