The detection of monkeypox virus DNA in wastewater samples in the Netherlands

Eline F de Jonge; Céline M Peterse; Jaap M Koelewijn; Anne-Merel R van der Drift; Rudolf F H J van der Beek; Erwin Nagelkerke; Willemijn J Lodder

doi:10.1016/j.scitotenv.2022.158265

The detection of monkeypox virus DNA in wastewater samples in the Netherlands

Sci Total Environ. 2022 Dec 15:852:158265. doi: 10.1016/j.scitotenv.2022.158265. Epub 2022 Aug 31.

Authors

Eline F de Jonge¹, Céline M Peterse¹, Jaap M Koelewijn¹, Anne-Merel R van der Drift¹, Rudolf F H J van der Beek¹, Erwin Nagelkerke¹, Willemijn J Lodder²

Affiliations

¹ Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
² Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands. Electronic address: willemijn.lodder@rivm.nl.

Abstract
in English, Dutch

Unlabelled Image

As of 18 August 2022, 1087 confirmed cases of monkeypox are reported in the Netherlands. Monkeypox virus likely ends up in wastewater because i) skin flakes from areas affected by the typical rash and scabbing may wash off, and ii) monkeypox virus has been detected in animal and human feces. Here we describe a method to qualitatively detect monkeypox virus DNA in wastewater, that may prove a valuable surveillance tool for outbreaks.

Keywords: Environmental surveillance; Monkeypox virus; Public health; WBE; Wastewater.

MeSH terms

DNA, Viral / genetics
Humans
Monkeypox virus* / genetics
Mpox (monkeypox)* / diagnosis
Netherlands
Wastewater

Substances

Waste Water
DNA, Viral

Abstract in English, Dutch

MeSH terms

Substances

Abstract
in English, Dutch