Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food

Yuechun Li; Han Zhang; Zhaowen Cui; Sijie Liu; Jingke Xu; Conghui Jia; Yaqian Chen; Lulu Wang; Jing Sun; Daohong Zhang; Mingqiang Zhu; Jianlong Wang

doi:10.1016/j.foodchem.2022.134012

Chemical staining enhanced Enzyme-linked immunosorbent assay for sensitive determination of Clenbuterol in food

Food Chem. 2023 Jan 30:400:134012. doi: 10.1016/j.foodchem.2022.134012. Epub 2022 Aug 27.

Authors

Yuechun Li¹, Han Zhang¹, Zhaowen Cui², Sijie Liu¹, Jingke Xu¹, Conghui Jia¹, Yaqian Chen¹, Lulu Wang¹, Jing Sun³, Daohong Zhang¹, Mingqiang Zhu⁴, Jianlong Wang⁵

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, China.
² College of Life Sciences, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, China.
³ Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xinning Road, Xining 810008, Qinghai, China.
⁴ College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling 712100, China.
⁵ College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling 712100, Shaanxi, China. Electronic address: wanglong79@nwsuaf.edu.cn.

PMID: 36055143
DOI: 10.1016/j.foodchem.2022.134012

Abstract

Exploring a novel strategy for strengthening the catalytic activity of enzyme facilitates the development of a sensitive enzyme-linked immunosorbent assay (ELISA). Herein, a chemical staining (CS) strategy was firstly discovered to possess the ability to directly improve the catalytic activity of horseradish peroxidase. Based on this discovery, coomassie brilliant blue was introduced into ELISA to establish a CS enhanced ELISA (CS-ELISA) to detect clenbuterol (CL) by simply staining monoclonal antibodies. Satisfactorily, the most important analytical parameters of CS-ELISA, including sensitivity (0.074 ng mL^-1) and linear range (0.2-2 ng mL^-1) were all improving 2-folds compared with conventional ELISA. Moreover, the CS-ELISA shows good applicability in the detection of CL in pork tenderloin samples. The proposed CS-ELISA shows various advantages, such as cost-effective, easily accessible, enhanced catalytic activity of enzyme, higher sensitivity, and broader linear range, providing a new insight into enhanced ELISA for food safety.

Keywords: Chemical Staining; Clenbuterol; Coomassie brilliant blue; Enzyme-linked immunosorbent assay; Food safety.

MeSH terms

Antibodies, Monoclonal
Clenbuterol* / analysis
Enzyme-Linked Immunosorbent Assay
Horseradish Peroxidase
Staining and Labeling

Substances

Antibodies, Monoclonal
Horseradish Peroxidase
Clenbuterol