Protocol for quantitative ethology on natural social interactions in Drosophila

Jing Ning; Zhou Li; Xingjiang Zhang; Junlong Wang; Dandan Chen; Qiong Liu; Yefeng Shao; Yi Sun

doi:10.1016/j.xpro.2022.101621

Protocol for quantitative ethology on natural social interactions in Drosophila

STAR Protoc. 2022 Aug 23;3(3):101621. doi: 10.1016/j.xpro.2022.101621. eCollection 2022 Sep 16.

Authors

Jing Ning¹, Zhou Li¹, Xingjiang Zhang¹, Junlong Wang¹, Dandan Chen², Qiong Liu², Yefeng Shao², Yi Sun³

Affiliations

¹ College of Life Sciences, Zhejiang University, Hangzhou, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, China; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China.
² Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, China; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China.
³ College of Life Sciences, Zhejiang University, Hangzhou, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China; Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, China; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China. Electronic address: sunyi@westlake.edu.cn.

Abstract

Quantitative ethology of social interactions between free-moving animals enables precise measurement of behavioral kinematics critical for various disciplines such as neuroscience. Here, we describe a set of tools for quantitative ethology of social interactions including the analysis pipeline SoAL, the training dataset SDPD, and the camera control software MIAS, along with experimental details. These tools are directly applicable for courtship behavior in Drosophila and can be used for other social interactions in other species after modification. For complete details on the use and execution of this protocol, please refer to Ning et al. (2022).

Keywords: Behavior; Bioinformatics; Model organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Data Collection
Drosophila
Ethology*
Neurosciences* / methods
Social Interaction

Associated data

figshare/10.6084/m9.figshare.17630585
figshare/10.6084/m9.figshare.17624888
figshare/10.6084/m9.figshare.19711729
figshare/10.6084/m9.figshare.19711738