Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice

Aftab Alam; Sarah Comer; Eric Levanduski; Prasenjit Dey

doi:10.1016/j.xpro.2022.101644

Fungal ablation and transplantation of specific fungal species into PDAC tumor-bearing mice

STAR Protoc. 2022 Aug 23;3(3):101644. doi: 10.1016/j.xpro.2022.101644. eCollection 2022 Sep 16.

Authors

Aftab Alam¹, Sarah Comer¹, Eric Levanduski¹, Prasenjit Dey²

Affiliations

¹ Department of Immunology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA.
² Department of Immunology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA. Electronic address: prasenjit.dey@roswellpark.org.

Abstract

Intratumor and gut mycobiome is linked to pancreatic ductal adenocarcinoma (PDAC) tumorigenesis; however, an optimal approach to culture and transplant fungus into mouse for in vivo studies is missing. This protocol describes culture steps of Alternaria alternata and Malassezia globosa and their subsequent transplantation into a PDAC mouse model via oral gavage. The utilization of the fungal culture method will allow for consistent growth and expansion of specific fungal species for downstream processing. For complete details on the use and execution of this protocol, please refer to Alam et al. (2022).

Keywords: Cancer; Immunology; Microbiology; Model organisms.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carcinogenesis / pathology
Carcinoma, Pancreatic Ductal* / surgery
Mice
Pancreatic Ducts / pathology
Pancreatic Neoplasms* / surgery

Abstract

Publication types

MeSH terms

Grants and funding