The complete chloroplast genome sequence of Nekemias cantoniensis (Hook. et Arn.) Planch 1873 (Vitaceae)

Yongjian Luo; Ru Wang; Qing Li; Jun Liu; Zhijun Deng

doi:10.1080/23802359.2022.2109436

The complete chloroplast genome sequence of Nekemias cantoniensis (Hook. et Arn.) Planch 1873 (Vitaceae)

Mitochondrial DNA B Resour. 2022 Aug 26;7(8):1536-1539. doi: 10.1080/23802359.2022.2109436. eCollection 2022.

Authors

Yongjian Luo^{1

2

3

4}, Ru Wang^{1

2

4}, Qing Li³, Jun Liu³, Zhijun Deng^{1

2

4}

Affiliations

¹ Hubei Key Laboratory of Biologic Resources Protection and Utilization (Hubei Minzu University), Enshi, PR China.
² The Plant Germplasm Resources Laboratory, School of Forestry and Horticulture, Hubei Minzu University, Enshi, PR China.
³ Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
⁴ Research Center for Germplasm Engineering of Characteristic Plant Resources in Enshi Prefecture (Hubei Minzu University), Enshi, PR China.

Abstract

Nekemias cantoniensis (Hook. et Arn.) Planch 1873 is a woody vine species native to South and Southwest China that is rich in flavonoids and also displays excellent pharmacological activities. The purpose of this study was to characterize the complete chloroplast (cp) genome of N. cantoniensis using Illumina pair-end sequencing data. In summary, the complete cp genome of N. cantoniensis exhibits a quadripartite structure with a length of 162,655 base pairs, including a large single-copy (LSC) region of 89,341 base pairs, a small single-copy (SSC) region of 19,076 base pairs, and two inverted repeats (IRs) regions of 27,119 base pairs. The overall GC content of the genome is 37.41%, while the corresponding values for the LSC, SSC, and IR regions are 34.75%, 32.89%, and 43.02%, respectively. The genome contains 137 genes, of which 87 are protein coding, 36 are tRNA coding, and eight are rRNA coding. Maximum-likelihood phylogenetic analyses revealed that N. cantoniensis was clustered with N. grossedentata.

Keywords: Chloroplast genome; maximum likelihood; phylogenetic relationship; small single-copy.

Grants and funding

The research in this paper was supported by the National Natural Science Foundation of China [31860073, 81303169, 31871716, 31371715], the Open Fund of Hubei Key Laboratory of Biologic Resources Protection and Utilization [PT012212], and the Science and Technology Program of Guangdong Province, China [2020B121201008, 2020B020209003, 2018B020202004]. The research in this paper was also supported by the Agricultural Science and Technology Cooperation Project of Foshan; Science and Technology Program of Guangzhou.