microRNA-27c negatively regulates NF-κB and IRF3 signaling pathway via targeting MITA in miiuy croaker

Xing Lv; Shiying Xin; Weiwei Zheng; Tianjun Xu; Yuena Sun

doi:10.1016/j.dci.2022.104522

microRNA-27c negatively regulates NF-κB and IRF3 signaling pathway via targeting MITA in miiuy croaker

Dev Comp Immunol. 2022 Dec:137:104522. doi: 10.1016/j.dci.2022.104522. Epub 2022 Aug 30.

Authors

Xing Lv¹, Shiying Xin¹, Weiwei Zheng¹, Tianjun Xu², Yuena Sun³

Affiliations

¹ Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
² Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, 201306, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306, China. Electronic address: tianjunxu@163.com.
³ Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, 201306, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306, China. Electronic address: yuenasun@163.com.

PMID: 36049570
DOI: 10.1016/j.dci.2022.104522

Abstract

As a non-coding RNA with regulatory functions, microRNAs(miRNAs) can regulate gene expression and participate in a variety of physiological and pathological processes. In recent years, although there have been many studies on miRNA, the regulation mechanisms of miRNA in teleost fish have not been fully elucidated. In this study, it was first predicted that MITA is the target of miR-27c through bioinformatics, and it was confirmed by dual fluorescence experiments. Then we found that miR-27c can inhibit the expression of MITA at the mRNA and protein levels, thereby promoting the NF-κB or IRF3 pathway. It is speculated that miR-27c plays an important role in the innate immunity of teleost fish. This study will help to further understand miRNAs regulatory mechanism in teleost fish.

Keywords: MITA; Miiuy croaker; Negatively regulate; microRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fish Proteins / metabolism
Gene Expression Regulation
MicroRNAs* / metabolism
NF-kappa B / metabolism
Perciformes*
RNA, Messenger / metabolism
Signal Transduction / genetics

Substances

Fish Proteins
MicroRNAs
NF-kappa B
RNA, Messenger