Genome Sequence of a Microvirus Recovered from Wastewater in Arizona, USA, in October 2020, Encodes a Previously Undescribed DNA-Binding Protein

Abriana Smith; Nicole Kaiser; Allan Yanez; Tyler Perleberg; Amir Elyaderani; Peter Skidmore; Sangeet Adhikari; Erin M Driver; Rolf U Halden; Arvind Varsani; Matthew Scotch; Temitope O C Faleye

doi:10.1128/mra.00337-22

Genome Sequence of a Microvirus Recovered from Wastewater in Arizona, USA, in October 2020, Encodes a Previously Undescribed DNA-Binding Protein

Microbiol Resour Announc. 2022 Oct 20;11(10):e0033722. doi: 10.1128/mra.00337-22. Epub 2022 Aug 31.

Authors

Abriana Smith^#¹, Nicole Kaiser^#¹, Allan Yanez², Tyler Perleberg², Amir Elyaderani¹, Peter Skidmore¹, Sangeet Adhikari^{2

3}, Erin M Driver², Rolf U Halden^{2

3

4}, Arvind Varsani⁵, Matthew Scotch^{1

2}, Temitope O C Faleye²

Affiliations

¹ College of Health Solutions, Arizona State University, Tempe, Arizona, USA.
² Biodesign Center for Environmental Health Engineering, Biodesign Institute, Arizona State University, Tempe, Arizona, USA.
³ School of Sustainable Engineering and the Built Environment, Arizona State University, Tempe, Arizona, USA.
⁴ OneWaterOneHealth, Arizona State University Foundation, Tempe, Arizona, USA.
⁵ Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, Arizona, USA.

^# Contributed equally.

Abstract

We describe the genome of Microvirus-AZ-2020, which was identified from wastewater in Arizona, USA, in October 2020. Microvirus-AZ-2020 belongs to subfamily Gokushovirinae and contains six (five known and one hypothetical) open reading frames (ORFs), each with >40 codons. HHPred analysis and Colabfold structure prediction suggest that the hypothetical ORF encodes a previously undescribed putative DNA-binding protein.

Grants and funding

U01LM013129/HHS | NIH | U.S. National Library of Medicine (NLM)