Method of manufacturing and staining microplastics for using in the biological experiments

Environ Sci Pollut Res Int. 2022 Sep;29(44):67450-67455. doi: 10.1007/s11356-022-22776-5. Epub 2022 Aug 30.

Abstract

Nowadays, plastic pollution attracts much attention both from society and scientists. The plastic pollution impact on the environment and human health requires assessment urgently, especially through experimental studies. However, such studies are still scarce because of the lack of standard methods for assessing their effects on living organisms. We have developed a process for manufacturing and staining PVC microparticles for using them in biological and ecological experiments. The electrospinning method has been used to manufacture PVC particles; their morphology and size have been analyzed. The obtained PVC particles are of narrow size range averaging 2-4 µm in diameter. They are successfully stained with the fluorescent dye Rhodamine B, which stands for the experiments performed in the seawater.

Keywords: Copepods; Electrospinning; Microplastic; Planktonic crustaceans; Polyvinyl chloride; Rhodamine B; Staining.

MeSH terms

  • Fluorescent Dyes
  • Humans
  • Microplastics*
  • Plastics
  • Polyvinyl Chloride
  • Staining and Labeling
  • Water Pollutants, Chemical* / analysis

Substances

  • Fluorescent Dyes
  • Microplastics
  • Plastics
  • Water Pollutants, Chemical
  • Polyvinyl Chloride