Co-60 gamma irradiation induced ovalbumin-glucose glycation and allergenicity reduction revealed by high-resolution mass spectrometry and ELISA assay

Guang-Xian Liu; Zong-Cai Tu; Hui Wang; Yueming Hu; Wen-Hua Yang

doi:10.1016/j.foodchem.2022.134013

Co-60 gamma irradiation induced ovalbumin-glucose glycation and allergenicity reduction revealed by high-resolution mass spectrometry and ELISA assay

Food Chem. 2023 Jan 15:399:134013. doi: 10.1016/j.foodchem.2022.134013. Epub 2022 Aug 24.

Authors

Guang-Xian Liu¹, Zong-Cai Tu², Hui Wang³, Yueming Hu⁴, Wen-Hua Yang⁵

Affiliations

¹ Institute of Agricultural Products Processing, Jiangxi Academy of Agricultural Sciences, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China.
² National Research and Development Center of Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
³ National Research and Development Center of Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China. Electronic address: wanghui00072@aliyun.com.
⁴ National Research and Development Center of Freshwater Fish Processing, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China.
⁵ College of Chemistry and Bioengineering, Yichun University, Yichun, Jiangxi 336000, China.

PMID: 36037695
DOI: 10.1016/j.foodchem.2022.134013

Abstract

Ovalbumin (OVA)-glucose mixture was treated with Co-60 irradiation at 0-25 kGy, and effects of irradiation on the glycation and allergenicity of OVA were investigated. Irradiation induced glycation between OVA and glucose, reflected in the significant increase of glycation sites from 3 to 14. Interestingly, OVA irradiated at 25 kGy had three new glycated peptides (568.78²⁺, 739.38²⁺ and 509.75²⁺). The degree of substitution per peptide molecule (DSP) of glycated peptides exhibited different trends with increasing irradiation dose. Particularly, glycated peptides 17-26, 55-60, 263-267 and 368-375 showed markedly decreased DSP values after irradiation at 20 and 25 kGy, which could be caused by the generation of Maillard reaction products (MRPs). MS/MS spectra suggested that neutral loss occurred in glycated arginine, whose structure was similar to MRPs. The IgG- and IgE-binding abilities of OVA significantly decreased with increasing irradiation dose, indicating that the protein allergenicity was reduced.

Keywords: Allergenicity; Glycated sites; Glycation; Irradiation; Ovalbumin.

MeSH terms

Allergens* / chemistry
Cobalt Radioisotopes*
Enzyme-Linked Immunosorbent Assay
Glucose
Ovalbumin / chemistry
Peptides / chemistry
Tandem Mass Spectrometry

Substances

Allergens
Cobalt Radioisotopes
Peptides
Cobalt-60
Ovalbumin
Glucose