Derivation and culturing of neural stem cells from human embryonic brain tissue

Yujin Suk; Agata Kieliszek; Daniel Mobilio; Chitra Venugopal; Sheila K Singh

doi:10.1016/j.xpro.2022.101628

Derivation and culturing of neural stem cells from human embryonic brain tissue

STAR Protoc. 2022 Aug 18;3(3):101628. doi: 10.1016/j.xpro.2022.101628. eCollection 2022 Sep 16.

Authors

Yujin Suk¹, Agata Kieliszek², Daniel Mobilio², Chitra Venugopal³, Sheila K Singh⁴

Affiliations

¹ Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Michael G DeGroote School of Medicine, McMaster University, Hamilton, ON L8S 4L8, Canada. Electronic address: yujin.suk@medportal.ca.
² Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.
³ Department of Surgery, McMaster University, Hamilton, ON L8S 4L8, Canada.
⁴ Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Department of Surgery, McMaster University, Hamilton, ON L8S 4L8, Canada. Electronic address: ssingh@mcmaster.ca.

Abstract

Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue. We describe the steps to dissociate embryonic brain tissue and culture hNSCs, followed by the procedure to expand hNSCs. These cells can be used for downstream applications including RNA-seq and omics studies. For complete details on the use and execution of this protocol, please refer to Venugopal et al. (2012b), Bakhshinyan et al. (2019), and Venugopal et al. (2012a).

Keywords: Cell culture; Cell isolation; Developmental biology; Health sciences; Neuroscience; Stem cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brain
Brain Neoplasms*
Humans
Neural Stem Cells*

Grants and funding

CIHR/Canada