A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming

Davi M Lyra-Leite; Óscar Gutiérrez-Gutiérrez; Meimei Wang; Yang Zhou; Lukas Cyganek; Paul W Burridge

doi:10.1016/j.xpro.2022.101560

A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming

STAR Protoc. 2022 Aug 18;3(3):101560. doi: 10.1016/j.xpro.2022.101560. eCollection 2022 Sep 16.

Authors

Davi M Lyra-Leite¹, Óscar Gutiérrez-Gutiérrez², Meimei Wang³, Yang Zhou³, Lukas Cyganek², Paul W Burridge⁴

Affiliations

¹ Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA; Center for Pharmacogenomics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
² Stem Cell Unit, Clinic for Cardiology and Pneumology, University Medical Center Göttingen, Göttingen, Germany; German Center for Cardiovascular Research (DZHK), Partner Site Göttingen, Göttingen, Germany.
³ Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA.
⁴ Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA; Center for Pharmacogenomics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. Electronic address: paul.burridge@northwestern.edu.

Abstract

The methods for the culture and cardiomyocyte differentiation of human embryonic stem cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex and uncontrolled systems to simplified and relatively robust protocols, using the knowledge and cues gathered at each step. HiPSC-derived cardiomyocytes have proven to be a useful tool in human disease modelling, drug discovery, developmental biology, and regenerative medicine. In this protocol review, we will highlight the evolution of protocols associated with hPSC culture, cardiomyocyte differentiation, sub-type specification, and cardiomyocyte maturation. We also discuss protocols for somatic cell direct reprogramming to cardiomyocyte-like cells.

Keywords: Cell Differentiation; Cell culture; Stem Cells.

Publication types

Review
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Human Embryonic Stem Cells*
Humans
Induced Pluripotent Stem Cells*
Myocytes, Cardiac

Grants and funding

R01 HL153220/HL/NHLBI NIH HHS/United States