A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes

STAR Protoc. 2022 Aug 18;3(3):101632. doi: 10.1016/j.xpro.2022.101632. eCollection 2022 Sep 16.

Abstract

Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021).

Keywords: Cell differentiation; Neuroscience; Stem cells; Tissue engineering.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Astrocytes
  • Cell Differentiation
  • Cells, Cultured
  • Humans
  • Induced Pluripotent Stem Cells*
  • Neurons