PpMYB52 negatively regulates peach bud break through the gibberellin pathway and through interactions with PpMIEL1

Yuzheng Zhang; Qiuping Tan; Ning Wang; Xiangguang Meng; Huajie He; Binbin Wen; Wei Xiao; Xiude Chen; Dongmei Li; Xiling Fu; Ling Li

doi:10.3389/fpls.2022.971482

PpMYB52 negatively regulates peach bud break through the gibberellin pathway and through interactions with PpMIEL1

Front Plant Sci. 2022 Aug 10:13:971482. doi: 10.3389/fpls.2022.971482. eCollection 2022.

Authors

Yuzheng Zhang^{1

2

3}, Qiuping Tan^{1

2

3}, Ning Wang^{1

2

3}, Xiangguang Meng^{1

2

3}, Huajie He^{1

2

3}, Binbin Wen^{1

2

3}, Wei Xiao^{1

2

3}, Xiude Chen^{1

2

3}, Dongmei Li^{1

2

3}, Xiling Fu^{1

2

3}, Ling Li^{1

2

3}

Affiliations

¹ College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, China.
² State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
³ Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai'an, China.

Abstract

Bud dormancy, which enables damage from cold temperatures to be avoided during winter and early spring, is an important adaptive mechanism of deciduous fruit trees to cope with seasonal environmental changes and temperate climates. Understanding the regulatory mechanism of bud break in fruit trees is highly important for the artificial control of bud break and the prevention of spring frost damage. However, the molecular mechanism underlying the involvement of MYB TFs during the bud break of peach is still unclear. In this study, we isolated and identified the PpMYB52 (Prupe.5G240000.1) gene from peach; this gene is downregulated in the process of bud break, upregulated in response to ABA and downregulated in response to GA. Overexpression of PpMYB52 suppresses the germination of transgenic tomato seeds. In addition, Y2H, Bimolecular fluorescence complementation (BiFC) assays verified that PpMYB52 interacts with a RING-type E3 ubiquitin ligase, PpMIEL1, which is upregulated during bud break may positively regulate peach bud break by ubiquitination-mediated degradation of PpMYB52. Our findings are the first to characterize the molecular mechanisms underlying the involvement of MYB TFs in peach bud break, increasing awareness of dormancy-related molecules to avoid bud damage in perennial deciduous fruit trees.

Keywords: ABA; GA; PpMIEL1; PpMYB52; bud break; peach.