Flavonoids affect the endophytic bacterial community in Ginkgo biloba leaves with increasing altitude

Shaodong Fu; Yan Deng; Kai Zou; Shuangfei Zhang; Xueduan Liu; Yili Liang

doi:10.3389/fpls.2022.982771

Flavonoids affect the endophytic bacterial community in Ginkgo biloba leaves with increasing altitude

Front Plant Sci. 2022 Aug 11:13:982771. doi: 10.3389/fpls.2022.982771. eCollection 2022.

Authors

Shaodong Fu^{1

2}, Yan Deng^{1

2}, Kai Zou³, Shuangfei Zhang^{1

2}, Xueduan Liu^{1

2}, Yili Liang^{1

2}

Affiliations

¹ School of Resource Processing and Bioengineering, Central South University, Changsha, China.
² Key Laboratory of Biometallurgy, Ministry of Education, Central South University, Changsha, China.
³ College of Advanced Materials Engineering, Jiaxing Nanhu University, Jiaxing, China.

Abstract

Altitude affects plant growth and metabolism, but the effect of altitude on plant endophytic microorganisms is still unclear. In this study, we selected 16 Ginkgo biloba trees to study the response of leaves' endophytes to flavonoids and altitude (from 530 m to 1,310 m). HPLC results showed that flavonoids in Ginkgo biloba leaves increased by more than 150% with attitude rising from 530 m to 1,310 m, which revealed a positive correlation with altitude. Ginkgo biloba might regulate the increased flavonoids in leaves to resist the increasing light intensity. 16S rDNA sequencing results showed that the endophytic bacterial communities of Ginkgo biloba at different altitudes significantly differed. Ginkgo leaf endophytes' alpha diversity decreased with increasing flavonoids content and altitude. The increased flavonoids might increase the environmental pressure on endophytes and affect the endophytic community in Ginkgo biloba leaves. The bacterial network in Ginkgo biloba leaves became more complex with increasing altitude, which might be one of the strategies of leaf endophytes to cope with increasing flavonoids. Metagenomes results predicted with PICRUSt showed that the abundance of flavonoid biosynthesis and photosynthesis genes were significantly decreased with the increase of flavonoid contents. High flavonoid content in leaves appeared to inhibit microbial flavonoid synthesis. Our findings indicate that altitude can modulate microbial community structure through regulating plant metabolites, which is important to uncovering the interaction of microbes, host and the environment.

Keywords: Ginkgo biloba; endophyte community; flavonoids; function genes; network.