Complete chloroplast genome and phylogenetic analysis of Bupleurum kaoi Liu, Chao, and Chuang, 1977: an endemic species in Taiwan

Chi-Chun Huang; Chao-Li Huang; Tsai-Wen Hsu; Li-Hui Chang; Kuo-Hsiang Hung; Wei-Kuang Wang

doi:10.1080/23802359.2022.2082892

Complete chloroplast genome and phylogenetic analysis of Bupleurum kaoi Liu, Chao, and Chuang, 1977: an endemic species in Taiwan

Mitochondrial DNA B Resour. 2022 Aug 22;7(8):1507-1509. doi: 10.1080/23802359.2022.2082892. eCollection 2022.

Authors

Chi-Chun Huang¹, Chao-Li Huang², Tsai-Wen Hsu¹, Li-Hui Chang¹, Kuo-Hsiang Hung³, Wei-Kuang Wang⁴

Affiliations

¹ Endemic Species Research Institute, Jiji, Nantou, Taiwan.
² Institute of Tropical Plant Sciences and Microbiology, National Cheng Kung University, Tainan, Taiwan.
³ Graduate Institute of Bioresources, Pingtung University of Science and Technology, Pingtung, Taiwan.
⁴ Department of Environmental Engineering and Science, Feng Chia University, Taichung, Taiwan.

Abstract

Bupleurum kaoi Liu, Chao, and Chuang is an endemic and endangered herb in Taiwan. In this study, the complete circular chloroplast genome of B. kaoi was reconstructed and annotated using Illumina sequencing. The genome size of B. kaoi is 155,938 bp, including a pair of inverted repeat regions (IRs: 26308 bp), separated by a large single-copy (LSC) region of 85,784 bp and a small single-copy (SSC) region of 17,538 bp. The GC content of the chloroplast genome is 37.6%. There are 113 different genes in the chloroplast genome of B. kaoi, including 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. A maximum-likelihood phylogenetic analysis showed that Bupleurum species is the monophyletic group, and B. kaoi belongs to subgenus Bupleurum and is closely related to B. scorzonerifolium.

Keywords: Bupleurum kaoi; chloroplast genome; phylogeny.

Grants and funding

This work was funded by the Council of Agriculture, Executive Yuan under a grant [number 110AS-1.3.2-ST-a8].