Metagenomics insight into bioaugmentation mechanism of Propionibacterium acidipropionici during anaerobic acidification of kitchen waste

Yi Zheng; Pan Wang; Xinyu Yang; Liya Zhao; Lianhai Ren; Ji Li

doi:10.1016/j.biortech.2022.127843

Metagenomics insight into bioaugmentation mechanism of Propionibacterium acidipropionici during anaerobic acidification of kitchen waste

Bioresour Technol. 2022 Oct:362:127843. doi: 10.1016/j.biortech.2022.127843. Epub 2022 Aug 27.

Authors

Yi Zheng¹, Pan Wang², Xinyu Yang³, Liya Zhao³, Lianhai Ren³, Ji Li⁴

Affiliations

¹ State Environmental Protection Key Laboratory of Food Chain Pollution Control, School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China; Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, China; College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China.
² State Environmental Protection Key Laboratory of Food Chain Pollution Control, School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China. Electronic address: wangpan@th.btbu.edu.cn.
³ State Environmental Protection Key Laboratory of Food Chain Pollution Control, School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China.
⁴ Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, China; College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China.

PMID: 36031136
DOI: 10.1016/j.biortech.2022.127843

Abstract

In the present study, a biochemical strategy for improving propionic acid production from kitchen waste acidification by bioaugmentation with Propionibacterium acidipropionici (P. acidipropionici) was investigated. When the inoculum of P. acidipropionici was 30% (w/w) of the seeding sludge, the propionic acid production increased by 79.57%. Further, bioaugmentation improved the relative abundance of Firmicute and Actinobacteria. The results of metagenomic analysis further reveal that the ATP-binding cassette (ABC) transporters and all related pathways of Propanoate metabolism (ko00640) were enriched when P. acidipropionici was added. For Propanoate metabolism, most functional genes involved in the conversion from Glycolysis / Gluconeogenesis (ko00010) to Propanoyl-CoA and conversion from Propanoyl-CoA to propionic acid were enhanced after bioaugmentation with P. acidipropionici, thereby promoting propionic acid production. As such, bioaugmentation with P. acidipropionici was effective in the anaerobic acidification of kitchen waste for propionic acid production.

Keywords: Anaerobic digestion; Functional genes; Metagenomic analysis; Microbial community structure; Propionic acid.

MeSH terms

Anaerobiosis
Coenzyme A / metabolism
Hydrogen-Ion Concentration
Metagenomics
Propionates* / metabolism
Propionibacteriaceae
Propionibacterium* / genetics
Propionibacterium* / metabolism

Substances

Propionates
Coenzyme A

Supplementary concepts

Acidipropionibacterium acidipropionici