Glycyrrhiza Polysaccharide Inhibits Pseudorabies Virus Infection by Interfering with Virus Attachment and Internalization

Changchao Huan; Yao Xu; Wei Zhang; Bo Ni; Song Gao

doi:10.3390/v14081772

Glycyrrhiza Polysaccharide Inhibits Pseudorabies Virus Infection by Interfering with Virus Attachment and Internalization

Viruses. 2022 Aug 14;14(8):1772. doi: 10.3390/v14081772.

Authors

Changchao Huan^{1

2

3}, Yao Xu^{1

2

3}, Wei Zhang^{1

2

3}, Bo Ni⁴, Song Gao^{1

2

3

5}

Affiliations

¹ Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
² Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.
³ Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, China.
⁴ Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China.
⁵ China Animal Health and Epidemiology Center, Qingdao 266011, China.

Abstract

Pseudorabies virus (PRV) is one of the most important pathogens causing serious diseases and leads to huge economic losses in the global swine industry. With the continuous emergence of PRV variants and the increasing number of cases of human infection, there is an urgent need to develop antiviral drugs. In this study, we discover that Glycyrrhiza polysaccharide (GCP) has anti-PRV infection activity in vitro, and 600 μg/mL GCP can completely block viral infection. The addition of GCP simultaneously with or after PRV infection had a significant inhibitory effect on PRV. Addition of GCP at different times of the virus life cycle mainly led to the inhibition of the attachment and internalization of PRV but does not affect viral replication and release. Our findings suggest that GCP has potential as a drug against PRV infection.

Keywords: Glycyrrhiza polysaccharide; antiviral activity; attachment; internalization; pseudorabies virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Glycyrrhiza*
Herpesvirus 1, Suid*
Humans
Polysaccharides / pharmacology
Pseudorabies* / drug therapy
Swine
Swine Diseases* / drug therapy
Virus Attachment
Virus Internalization

Substances

Polysaccharides

Grants and funding

This research was funded by the National Natural Science Foundation of China (31902253), Jiangsu Provincial Natural Science Fund for Excellent Young Scholars (BK20220114), China Postdoctoral Science Foundation (2018M632399), Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the earmarked fund of Jiangsu Agricultural Industry Technology System, and the Top-Level Talents Support Program of Yangzhou University.