Transcriptomic Analysis of the Porcine Gut in Response to Heat Stress and Dietary Soluble Fiber from Beet Pulp

Minju Kim; Eugeni Roura; Yohan Choi; Joeun Kim

doi:10.3390/genes13081456

Transcriptomic Analysis of the Porcine Gut in Response to Heat Stress and Dietary Soluble Fiber from Beet Pulp

Genes (Basel). 2022 Aug 16;13(8):1456. doi: 10.3390/genes13081456.

Authors

Minju Kim¹, Eugeni Roura¹, Yohan Choi², Joeun Kim²

Affiliations

¹ Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD 4072, Australia.
² Swine Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, Korea.

Abstract

This study aimed to investigate the impact of heat stress (HS) and the effects of dietary soluble fiber from beet pulp (BP) on gene expression (differentially expressed genes, DEGs) of the porcine jejunum. Out of the 82 DEGs, 47 genes were up-regulated, and 35 genes were downregulated between treatments. The gene ontology (GO) enrichment analysis showed that the DEGs were related mainly to the actin cytoskeleton organization and muscle structure development in biological processes, cytoplasm, stress fibers, Z disc, cytoskeleton, and the extracellular regions in cellular composition, and actin binding, calcium ion binding, actin filament binding, and pyridoxal phosphate binding in the molecular function. The KEGG pathway analysis showed that the DEGs were involved in hypertrophic cardiomyopathy, dilated cardiomyopathy, vascular smooth muscle contraction, regulation of actin cytoskeleton, mucin type O-glycan biosynthesis, and African trypanosomiasis. Several of the genes (HSPB6, HSP70, TPM1, TAGLN, CCL4) in the HS group were involved in cellular oxidative stress, immune responses, and cellular differentiation. In contrast, the DEGs in the dietary BP group were related to intestinal epithelium integrity and immune response to pathogens, including S100A2, GCNT3, LYZ, SCGB1A1, SAA3, and ST3GAL1. These findings might help understand the HS response and the effect of dietary fiber (DF) regarding HS and be a valuable reference for future studies.

Keywords: RNA-Seq; beet pulp; gene expression; heat stress; pig; small intestine; soluble fiber.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Beta vulgaris* / genetics
Dietary Fiber
Gene Expression Profiling
Heat-Shock Response / genetics
Swine
Transcriptome

Substances

Dietary Fiber

Grants and funding

This research was funded by the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01479603), Rural Development Administration, Korea.