Understanding the Protective Role of IL-17 During Oropharyngeal Candidiasis

Jessica Saul-McBeth; Heather R Conti

doi:10.1007/978-1-0716-2549-1_25

Understanding the Protective Role of IL-17 During Oropharyngeal Candidiasis

Methods Mol Biol. 2022:2542:361-373. doi: 10.1007/978-1-0716-2549-1_25.

Authors

Jessica Saul-McBeth¹, Heather R Conti²

Affiliations

¹ The University of Toledo, Department of Biological Sciences, Toledo, OH, USA.
² The University of Toledo, Department of Biological Sciences, Toledo, OH, USA. heather.conti@utoledo.edu.

PMID: 36008677
DOI: 10.1007/978-1-0716-2549-1_25

Abstract

Oropharyngeal candidiasis is an opportunistic mucosal infection caused predominantly by Candida albicans. While healthy individuals are protected, susceptibility is associated with immunodeficiency. In particular, patients with defects related to T helper-17 (Th17) cells and interleukin (IL)-17 signaling are highly susceptible to mucocutaneous forms of candidiasis. Since mice are naïve to Candida albicans, induction of oropharyngeal candidiasis enables a thorough understanding of IL-17 and its related immune components during acute infection. Here we describe a murine model of oropharyngeal candidiasis. This protocol allows for translatable and reproducible infection with results that can be obtained between 2 and 5 days following infection.

Keywords: Candida albicans; Interleukin-17; Murine model; Oral candidiasis; Oropharyngeal candidiasis; Th17.

MeSH terms

Animals
Candida albicans
Candidiasis, Oral* / metabolism
Candidiasis, Oral* / pathology
Interleukin-17 / metabolism*
Mice
Th17 Cells

Substances

Il17a protein, mouse
Interleukin-17