Proteomics reveals the enhancing mechanism for eliminating toxic hydroxylamine from water by nanocompartments containing hydroxylamine oxidase

Abstract

Hydroxylamine (NH₂OH) is a potentially toxic pollutant when it is present in water, as it can damage both bacteria and the human body. It is still difficult to eliminate the toxic NH₂OH in water. Here, we showed that the model bacterium (Escherichia coli) with nanocompartments encapsulated with hydroxylamine oxidase (HAO) can remove NH₂OH from water. In addition, the removal efficiency of NH₂OH by genetically modified bacteria (with HAO-nanocompartments) was 3.87 mg N L^-1 h^-1, and that of wild-type bacteria (without HAO-nanocompartments) was only 1.86 mg N L^-1 h^-1. Label-free quantitative proteomics indicated that the nanocompartments containing HAO enhanced bacterial activity by inducing the up-regulation of proteins involved in stress and stimulus responses, and decreased their intracellular NH₂OH concentration. Moreover, the synthesis of proteins involved in energy metabolism, gene expression, and other processes in bacterial was enhanced under hydroxylamine stress, and these changes increased the resistance of bacterial to NH₂OH. This work can aid our understanding of the toxic effects of NH₂OH on bacteria as well as the development of new approaches to eliminate NH₂OH in water.

Keywords: Hydroxylamine; Hydroxylamine oxidase; Nanocompartments; Proteomics.