Molecular imprinting-aptamer techniques exhibit the advantages of molecular imprinting and aptamer technology. Hybrids of molecularly imprinted polymer-aptamer (MIP-aptamer) prepared by this technique have higher stability, binding affinity and superior selectivity than conventional molecularly imprinted polymers or aptamers. In recent years, molecular imprinting-aptamer technologies have attracted considerable interest for the selective recognition of target molecules in complex sample matrices and have been used in molecular recognition such as antibiotics, proteins, viruses and pesticides. This review introduced the development of molecular imprinting-aptamer-combining technologies and summarized the mechanism of MIP-aptamer formation. Meanwhile, we discussed the challenges in preparing MIP-aptamer. Finally, we summarized the application of MIP-aptamer to the molecular recognition in disease diagnosis, environmental analysis, food safety and other fields.
Keywords: aptamer; molecular recognition; molecularly imprinted polymer.