The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper

Zhi-Yong Tao; Pei-Yi Zhang; Lu Zhang; Chun-Cao Li; Rui Hu; Han-Wu Zhu; Bei Zhou; Kai Wu; Ling-Xu Li; Da-Wei Yao; Yu-Jie Cao; Dao-Jin Wang; Chen-Chen Zheng; Run-Qi Fang; Xiu-Min Hua; Yi-Xuan Ni; Xiao-Xia Jin; Hui Xia; Qiang Fang

doi:10.1155/2022/5715436

The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper

J Trop Med. 2022 Aug 13:2022:5715436. doi: 10.1155/2022/5715436. eCollection 2022.

Authors

Zhi-Yong Tao^{1

2}, Pei-Yi Zhang^{1

2}, Lu Zhang^{1

2}, Chun-Cao Li^{1

2}, Rui Hu^{1

2}, Han-Wu Zhu³, Bei Zhou⁴, Kai Wu⁵, Ling-Xu Li⁶, Da-Wei Yao⁶, Yu-Jie Cao^{1

2}, Dao-Jin Wang², Chen-Chen Zheng², Run-Qi Fang², Xiu-Min Hua^{1

2}, Yi-Xuan Ni^{1

2}, Xiao-Xia Jin^{1

2}, Hui Xia^{1

2}, Qiang Fang^{1

2}

Affiliations

¹ Department of Microbiology and Parasitology, Bengbu Medical College, 2600 Donghai Avenue, Bengbu 233030, China.
² Anhui Key Laboratory of Infection and Immunology, Bengbu Medical College, 2600 Donghai Avenue, Bengbu 233030, China.
³ Chenzhou Center for Disease Control and Prevention, Chenzhou 423000, Hunan, China.
⁴ College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China.
⁵ Wuhan Center for Disease Control and Prevention, Wuhan 430022, Hubei, China.
⁶ College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, China.

Abstract

Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting Plasmodium results showed that the minimum detection limit of the 4 filter papers was 1 × 10² parasites/μL, and the positive rates of 20 PCR kits ranged from 0% to 100%. PCR results were satisfactory for detecting Plasmodium falciparum (P. falciparum) and Plasmodium. vivax (P. vivax) in archived DBS samples and Babesia gibsoni (B. gibsoni) in fresh pet DBS samples. Our results provided a useful reference for the detection of blood pathogens with DBS samples and direct PCR, especially for screening the cost-efficacy combination of filter paper and PCR kit in resource-limited areas.