A clickable AIEgen for visualization of macrophage-microbe interaction

Zicong Zhang; Wei He; Ziwei Deng; Yanling Liu; Haifei Wen; Yucheng Wang; Ziyue Ye; Ryan Tsz Kin Kwok; Zijie Qiu; Zheng Zhao; Ben Zhong Tang

doi:10.1016/j.bios.2022.114614

A clickable AIEgen for visualization of macrophage-microbe interaction

Biosens Bioelectron. 2022 Nov 15:216:114614. doi: 10.1016/j.bios.2022.114614. Epub 2022 Aug 18.

Authors

Zicong Zhang¹, Wei He², Ziwei Deng¹, Yanling Liu¹, Haifei Wen¹, Yucheng Wang¹, Ziyue Ye¹, Ryan Tsz Kin Kwok³, Zijie Qiu¹, Zheng Zhao⁴, Ben Zhong Tang⁵

Affiliations

¹ School of Science and Engineering, Shenzhen Key Laboratory of Functional Aggregate Materials, The Chinese University of Hong Kong, Shenzhen, Guangdong, 518172, China.
² Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China; HKUST-Shenzhen Research Institute, South Area Hi-Tech Park, Nanshan, Shenzhen, Guangdong Province, 518057, China.
³ Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
⁴ School of Science and Engineering, Shenzhen Key Laboratory of Functional Aggregate Materials, The Chinese University of Hong Kong, Shenzhen, Guangdong, 518172, China; HKUST-Shenzhen Research Institute, South Area Hi-Tech Park, Nanshan, Shenzhen, Guangdong Province, 518057, China. Electronic address: zhaozheng@cuhk.edu.cn.
⁵ School of Science and Engineering, Shenzhen Key Laboratory of Functional Aggregate Materials, The Chinese University of Hong Kong, Shenzhen, Guangdong, 518172, China; Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. Electronic address: tangbenz@cuhk.edu.cn.

PMID: 35995026
DOI: 10.1016/j.bios.2022.114614

Abstract

Visualization of immunocyte-microbe interaction is of great importance to reveal the physiological role and working mechanism of innate and adaptive immune system. The lack of rapid and stable microbial labeling platform and insufficient understanding of macrophage-microbe interaction may delay precautions that could be made. In this contribution, a clickable AIEgen, CDPP-NCS, containing a cationic pyridinium moiety for targeting bacteria and an isothiocyanate moiety for covalently bonding with amine groups, is successfully developed. With the advantages of excellent photostability and rapid bioconjugation with amine groups on the bacterial envelope, the processes of macrophage-bacterium interactions with subcellular resolution has been successfully captured using this clickable AIE probe. Therefore, the new clickable AIEgen is a powerful tool to study the interaction between cell and bacterium.

Keywords: Aggregation-induced emission; Bioimaging; Fluorescent probe; Macrophage-microbe interaction.

MeSH terms

Amines
Bacteria
Biosensing Techniques*
Fluorescent Dyes
Isothiocyanates
Macrophages

Substances

Amines
Fluorescent Dyes
Isothiocyanates