The role of tetraspanins pan-cancer

Runzhi Huang; Hanlin Sun; Ruoyi Lin; Jie Zhang; Huabin Yin; Shuyuan Xian; Man Li; Siqiao Wang; Zhenyu Li; Yannan Qiao; Meiyun Jiang; Penghui Yan; Tong Meng; Zongqiang Huang

doi:10.1016/j.isci.2022.104777

The role of tetraspanins pan-cancer

iScience. 2022 Jul 19;25(8):104777. doi: 10.1016/j.isci.2022.104777. eCollection 2022 Aug 19.

Authors

Runzhi Huang^{1

2

3

4}, Hanlin Sun^{1

5}, Ruoyi Lin⁴, Jie Zhang^{2

3

4}, Huabin Yin⁶, Shuyuan Xian⁴, Man Li¹, Siqiao Wang⁴, Zhenyu Li⁴, Yannan Qiao¹, Meiyun Jiang¹, Penghui Yan¹, Tong Meng^{6

7}, Zongqiang Huang¹

Affiliations

¹ Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Zhengzhou, 450052, China.
² Division of Spine Surgery, Department of Orthopaedics, Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China.
³ Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University), Ministry of Education, Shanghai, 200065, China.
⁴ Tongji University School of Medicine, 1239 Siping Road, Shanghai, 200092, China.
⁵ Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, 1 East Jianshe Road, Zhengzhou, 450052, China.
⁶ Department of Orthopedics, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, 100 Haining Road, Shanghai, China.
⁷ Tongji University Cancer Center, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China.

Abstract

Tetraspanins (TSPANs) are a class of four-transmembrane segmented proteins. The precise functions of TSPANs and their roles in pan-cancer are unclear. In this work, we analyzed TSPAN1, TSPAN10, TSPAN11, TSPAN12, TSPAN13, TSPAN14, TSPAN15, TSPAN16, TSPAN17, TSPAN18, TSPAN18-AS1, TSPAN19, TSPAN2, TSPAN3, TSPAN31, TSPAN32, TSPAN33, TSPAN4, TSPAN5, TSPAN6, TSPAN7, TSPAN8, TSPAN9, TSPAN9-IT1 (24 TSPAN family genes) in relation to tumor characteristics from 11,057 TCGA samples across 33 cancer types. On 24 TSPAN family genes, multidimensional studies were conducted, including gene differential expression, immunological subtype analysis, clinical analysis, stemness indices analysis, drug sensitivity analysis, alteration analysis, and multi-omics validation (including ATAC-seq validation, single-cell sequencing validation, and other external validation). Genes were differentially expressed in 33 cancers, and several of them showed high consistency in terms of tumor characteristics. In particular, the potential roles of TSPAN15 and TSPAN1 in cancer deserve further attention.

Keywords: Cancer; Cancer systems biology; Transcriptomics.