Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)

Matthew Alonzo; Javier Contreras; Shiqiao Ye; Hui Lin; Lumariz Hernandez-Rosario; Kim L McBride; Karen Texter; Vidu Garg; Ming-Tao Zhao

doi:10.1016/j.scr.2022.102892

Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS)

Stem Cell Res. 2022 Oct:64:102892. doi: 10.1016/j.scr.2022.102892. Epub 2022 Aug 9.

Authors

Matthew Alonzo¹, Javier Contreras¹, Shiqiao Ye¹, Hui Lin¹, Lumariz Hernandez-Rosario¹, Kim L McBride², Karen Texter³, Vidu Garg², Ming-Tao Zhao⁴

Affiliations

¹ Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.
² Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH, USA.
³ The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH, USA.
⁴ Center for Cardiovascular Research, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH, USA; The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH, USA. Electronic address: Mingtao.Zhao@nationwidechildrens.org.

Abstract

Hypoplastic left heart syndrome (HLHS) is a severe congenital heart defect characterized by underdeveloped structures on the left side of the heart, including hypoplasia of the left ventricle and stenosis or atresia of the aortic and mitral valves. Here, we generated an iPSC line from the peripheral blood mononuclear cells of a male patient with HLHS through Sendai virus-mediated transfection of 4 Yamanaka factors. This iPSC line exhibited normal morphology, expressed pluripotency markers, had a normal karyotype, and could differentiate into cells of three germ layers. This iPSC line can be used for studying cellular and developmental etiologies of HLHS.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Heart Defects, Congenital*
Heart Ventricles
Humans
Hypoplastic Left Heart Syndrome*
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Male

Grants and funding

R01 HL155282/HL/NHLBI NIH HHS/United States