Two smart coumarin-based fluorescent probes with AIE effect for sensing ClO- and imaging in living cells

Abstract

It's worth noting that detect effective methods for tracking ClO^- could help us uncover the function of ClO^- in living systems. Here, two coumarin-based probes, named (E)-3-(1-hydrazonoethyl)-2H-chromen-2-one (1A) and 3-((E)-1-(((E)-(2,3-dihydro-1H-imidazol-4-yl)methylene)-hydrazono)ethyl)- 2H-chromen-2-one (1B) with aggregation-induced emission (AIE) effect in Tris-HCl (pH = 7.2) buffer solution were synthesized and used for sensing ClO^- selectivity. 1A and 1B responded to ClO^- through the oxidation hydrolysis effect. The mechanism was further verified by HR-MS and DFT calculation. Cell imaging indicated that 1A and 1B were good membrane permeability with low toxicity to HEK293T, and expected to be used to detect ClO^- in cells.

Keywords: Aggregation-Induced Emission (AIE); Cell imaging; ClO(-) recognition; DFT calculation; Fluorescent probe.