Clonal dissemination of carbapenem-resistant Klebsiella pneumoniae ST16 co-producing NDM-1 and OXA-232 in Thailand

Ryuichiro Abe; Yukihiro Akeda; Dan Takeuchi; Noriko Sakamoto; Yo Sugawara; Norihisa Yamamoto; Anusak Kerdsin; Yuki Matsumoto; Daisuke Motooka; Warawut Leolerd; Pitak Santanirand; Masato Suzuki; Keigo Shibayama; Kazunori Tomono; Tetsuya Iida; Shigeyuki Hamada

doi:10.1093/jacamr/dlac084

Clonal dissemination of carbapenem-resistant Klebsiella pneumoniae ST16 co-producing NDM-1 and OXA-232 in Thailand

JAC Antimicrob Resist. 2022 Aug 16;4(4):dlac084. doi: 10.1093/jacamr/dlac084. eCollection 2022 Aug.

Authors

Ryuichiro Abe¹, Yukihiro Akeda¹, Dan Takeuchi¹, Noriko Sakamoto¹, Yo Sugawara¹, Norihisa Yamamoto², Anusak Kerdsin³, Yuki Matsumoto⁴, Daisuke Motooka⁴, Warawut Leolerd⁵, Pitak Santanirand⁵, Masato Suzuki⁶, Keigo Shibayama⁷, Kazunori Tomono², Tetsuya Iida¹, Shigeyuki Hamada¹

Affiliations

¹ Japan-Thailand Research Collaboration Center on Emerging and Re-emerging Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
² Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.
³ Faculty of Public Health, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
⁴ Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
⁵ Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
⁶ Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
⁷ Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.

Abstract

Background: Klebsiella pneumoniae ST258 and ST11 carrying bla _KPC are among the most widespread carbapenem-resistant K. pneumoniae strains worldwide. Our carbapenem-resistant Enterobacteriaceae surveillance in Thailand revealed a nationwide dissemination of K. pneumoniae ST16 isolates carrying bla _NDM-1 and bla _OXA-232.

Objectives: To analyse the genomic details of this nationwide dissemination by focusing on plasmids and virulence factors.

Methods: Using WGS data of 119 K. pneumoniae ST16 isolates carrying bla _NDM-1 obtained in our previous surveillance study, clonality of chromosomes and plasmids of the isolates with carriage of virulence factors was evaluated.

Results: Of the 119 isolates, 111 carried plasmid pKP151_NDM1, and all 104 isolates harbouring bla _OXA-232 carried plasmid pKP151_OXA232. These 104 K. pneumoniae ST16 isolates showing chromosomal clonality possessed both pKP151_NDM1 and pKP151_OXA232, demonstrating clonal dissemination of K. pneumoniae ST16 with these plasmids. The isolates had essentially similar virulence factors as those of K. pneumoniae ST16 clones carrying bla _KPC, which were recently reported as highly invasive clones in Brazil.

Conclusions: The potential global dissemination of these invasive clones with resistance to several antibiotics highlights the importance of appropriate monitoring and strict standard precautions.