Evaluation of the Rapid Sepsityper protocol and specific MBT-Sepsityper module for the identification of bacteremia and fungemia using Bruker Biotyper MALDI-TOF MS

Hsiu-Hsien Lin; Kun-Hao Tseng; Ni Tien; Yu-Tzu Lin; Jiaxin Yu; Po-Ren Hsueh; Der-Yang Cho

doi:10.1016/j.jmii.2022.07.005

Evaluation of the Rapid Sepsityper protocol and specific MBT-Sepsityper module for the identification of bacteremia and fungemia using Bruker Biotyper MALDI-TOF MS

J Microbiol Immunol Infect. 2022 Dec;55(6 Pt 2):1330-1333. doi: 10.1016/j.jmii.2022.07.005. Epub 2022 Aug 5.

Authors

Hsiu-Hsien Lin¹, Kun-Hao Tseng¹, Ni Tien², Yu-Tzu Lin³, Jiaxin Yu⁴, Po-Ren Hsueh⁵, Der-Yang Cho⁶

Affiliations

¹ Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
² Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan; Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.
³ Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.
⁴ AI Innovation Center, China Medical University Hospital, Taichung, Taiwan.
⁵ Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan; Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan; School of Medicine, China Medical University, Taichung, Taiwan. Electronic address: hsporen@gmail.com.
⁶ Department of Neurosurgery, China Medical University Hospital, Taichung, Taiwan.

PMID: 35981943
DOI: 10.1016/j.jmii.2022.07.005

Abstract

The rapid identification method, the Rapid Sepsityper protocol with a specific MBT-Sepsityper module (Bruker Daltonics), based on the MALDI Biotyper platform, accurately identified 93.5% (116/124) of microorganisms at the species level in the 124 flagged blood culture samples from patients with monomicrobial bloodstream infections. Gram-negative bacilli (95.6%, 43/45) had a higher identification rate than Gram-positive cocci (93.3%, 70/75) and yeasts (75%, 3/4). The Rapid Sepsityper protocol displayed poor identification performance for polymicrobial samples.

Keywords: Bacteremia; Bruker Biotyper MALDI-TOF MS; Fungemia; Identification; Rapid Sepsityper Protocol.

MeSH terms

Bacteremia* / diagnosis
Blood Culture / methods
Fungemia* / diagnosis
Gram-Negative Bacteria
Humans
Sepsis* / diagnosis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods