Objective: To detect the expression of multiple myeloma-associated antigen (MMSA)-8 and MMSA-1 in bone marrow mononuclear cells of patients with acute myeloid leukemia, and explore their roles in acute myeloid leukemia.
Methods: A total of 83 patients with M2 acute myeloid leukemia in our hospital from January 2019 to January 2020 were selected as research group, during the same period, 15 patients diagnosed iron deficiency anemia were selected as control group. Real-time fluorescence quantitative PCR was used to detect the levels of MMSA-8 and MMSA-1 in bone marrow mononuclear cells. Patients in the research group were divided into remission and non remission according to the clinical curative effect, and were divided into good prognosis, medium prognosis, and poor prognosis according to the prognosis. The relationship between MMSA-8, MMSA-1 and clinical efficacy, prognosis was analyzed. In addition, the general data of patients in the research group were collected, including white blood cell count (WBC), hemoglobin (Hb), platelet count (PLT), and percentage of bone marrow progenitor cells at admission. Pearson method was used to analyze the correlation between MMSA-8, MMSA-1 and clinical data, and MMSA-8 and MMSA-1.
Results: The analysis results about mRNA levels of MMSA-8 and MMSA-1 in bone marrow mononuclear cells of patients showed that patients in the research group were significantly higher than those in the control group (P<0.05); In the research group, patients without remission were also significantly higher than those with remission, as well as those with medium and poor prognosis than with good prognosis, while only mRNA level of MMSA-1 in patients with poor prognosis was significantly higher than those with medium prognosis (P<0.05). Pearson analysis showed that MMSA-8, MMSA-1 were positively correlated with WBC (r=0.468, r=0.516), and MMSA-8 was positively correlated with MMSA-1 (r=0.318).
Conclusion: The levels of MMSA-8 and MMSA-1 in bone marrow mononuclear cells of patients with M2 acute myeloid leukemia are increased, which are closely related to the occurrence and development of the disease, and have certain value for the prognosis.
题目: M2型急性髓系白血病患者骨髓中MMSA-8、MMSA-1表达及临床意义.
目的: 检测急性髓系白血病患者骨髓单个核细胞中多发性骨髓瘤相关抗原（MMSA）-8、MMSA-1表达情况，探讨二者在急性髓系白血病中的临床意义。.
方法: 纳入2019年1月-2020年1月本院M2型急性髓系白血病患者83例为研究组，同期血液科诊断为单纯缺铁性贫血患者15例为对照组，实时荧光定量PCR检测骨髓单个核细胞中MMSA-8、 MMSA-1表达水平。将研究组患者根据疗效分为缓解、未缓解，根据预后状况分为预后良好、预后中等和预后不良，分析MMSA-8、MMSA-1与疗效、预后的关系。另收集研究组患者的一般资料，包括入院时血常规白细胞数、血红蛋白、血小板数和骨髓原始细胞百分比，Pearson法分析MMSA-8、MMSA-1与患者临床资料以及MMSA-8与MMSA-1的相关性。.
结果: 对患者骨髓单个核细胞中MMSA-8、MMSA-1 mRNA水平分析结果显示，研究组患者明显高于对照组患者（P<0.05）；研究组中未缓解患者明显高于缓解患者（P<0.05），预后中等、预后不良患者均明显高于预后良好患者（P<0.05），预后不良患者仅MMSA-1 mRNA水平明显高于预后中等患者（P<0.05）。Pearson法分析结果显示，研究组患者MMSA-8、MMSA-1与白细胞数呈正相关（r=0.468，r=0.516），MMSA-8与MMSA-1呈正相关（r=0.318）。.
结论: M2型急性髓系白血病患者骨髓单个核细胞中MMSA-8、MMSA-1水平升高，二者均与疾病发生发展关系密切，且对预后有一定预测价值。.
Keywords: acute myeloid leukemia; bone marrow; multiple myeloma-associated antigen-1; multiple myeloma-associated antigen-8.