Prevalence of Multidrug-Resistant CTX-M Extended Spectrum Beta-Lactamase-Producing Escherichia coli From Different Bovine Faeces in China

Xiaojuan Wei; Weiwei Wang; Ningning Lu; Lingyu Wu; Zhen Dong; Bing Li; Xuzheng Zhou; Fusheng Cheng; Kairen Zhou; Haijian Cheng; Hongmei Shi; Jiyu Zhang

doi:10.3389/fvets.2022.738904

Prevalence of Multidrug-Resistant CTX-M Extended Spectrum Beta-Lactamase-Producing Escherichia coli From Different Bovine Faeces in China

Front Vet Sci. 2022 Aug 1:9:738904. doi: 10.3389/fvets.2022.738904. eCollection 2022.

Authors

Xiaojuan Wei^{1

2

3}, Weiwei Wang^{1

2

3}, Ningning Lu^{1

2

3}, Lingyu Wu^{1

2

3}, Zhen Dong^{1

2

3}, Bing Li^{1

2

3}, Xuzheng Zhou^{1

2

3}, Fusheng Cheng^{1

2

3}, Kairen Zhou^{1

2

3}, Haijian Cheng⁴, Hongmei Shi⁵, Jiyu Zhang^{1

2

3}

Affiliations

¹ Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Science (CAAS), Lanzhou, China.
² Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, China.
³ Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, China.
⁴ Shandong Institute of Animal Science and Veterinary Medicine, Jinan, China.
⁵ Gannan Tibetan Autonomous Prefecture Institute of Animal Husbandry Science, Gannan, China.

Abstract

CTX-M extended spectrum beta-lactamase-producing Escherichia coli cause severe health hazards in livestock breeding. To date, little is known about antibiotic resistance differences among bacterial isolates from yaks, cows, and beef cattle; therefore, the aims of this study were to analyse the prevalence of CTX-M-producing E. coli in yak, beef cattle, and dairy cattle feces from different provinces in China. A total of 790 fecal samples from yaks, beef cattle, and dairy cows were used. Among all the samples, 523 non duplicate E. coli isolates were identified, and 29.6% of samples harbored CTX-M producers. The results showed that these E. coli strains harbored 15 clusters of CTX-M genes: CTX-M-79, CTX-M-55, CTX-M-15, CTX-M-14, CTX-M-28, CTX-M-179, CTX-M-65, CTX-M-24, CTX-M-27, CTX-M-102, CTX-M-105, CTX-M-173, CTX-M-238, CTX-M-196, and CTX-M-10. The dominant resistance genes were CTX-M-15, CTX-M-14, and CTX-M-55. Moreover, the distribution of CTX-M genes was related to geographical region. Based on the above findings, we reasoned that bovines are potential reservoirs of antibiotic resistance, and this problem should be given adequate attention.

Keywords: E. coli; beef cattle; blaCTX-M; dairy cattle; feces; yak.