Synthesis of the l- and d-SH2 domain of the leukaemia oncogene Bcr-Abl

Nina Schmidt; Frank Abendroth; Olalla Vázquez; Oliver Hantschel

doi:10.1039/d2cb00108j

Synthesis of the l- and d-SH2 domain of the leukaemia oncogene Bcr-Abl

RSC Chem Biol. 2022 Jul 6;3(8):1008-1012. doi: 10.1039/d2cb00108j. eCollection 2022 Aug 3.

Authors

Nina Schmidt¹, Frank Abendroth², Olalla Vázquez^{2

3}, Oliver Hantschel¹

Affiliations

¹ Institute of Physiological Chemistry, University of Marburg 35032 Marburg Germany oliver.hantschel@uni-marburg.de.
² Faculty of Chemistry, University of Marburg 35032 Marburg Germany olalla.vazquez@staff.uni-marburg.de.
³ Center for Synthetic Microbiology (SYNMIKRO), University of Marburg 35032 Marburg Germany.

Abstract

The d- and l-versions of the Bcr-Abl SH2 domain (12.7 kDa) were synthesized. Key optimizations included pseudoproline incorporation, N-terminal hydrophilic tail addition and mild N-acetoxy succinimide acetylation. Their folding and activity are as for the recombinant protein. Our results will enable engineering of mirror-image monobody antagonists of the central oncoprotein Bcr-Abl.