Mesoporous sodium four-coordinate aluminosilicate nanoparticles modulate dendritic cell pyroptosis and activate innate and adaptive immunity

Jie Tang; Yang Yang; Jingjing Qu; Wenhuang Ban; Hao Song; Zhengying Gu; Yannan Yang; Larry Cai; Shevanuja Theivendran; Yue Wang; Min Zhang; Chengzhong Yu

doi:10.1039/d1sc05319a

Mesoporous sodium four-coordinate aluminosilicate nanoparticles modulate dendritic cell pyroptosis and activate innate and adaptive immunity

Chem Sci. 2022 Jul 20;13(29):8507-8517. doi: 10.1039/d1sc05319a. eCollection 2022 Jul 29.

Authors

Jie Tang¹, Yang Yang¹, Jingjing Qu¹, Wenhuang Ban¹, Hao Song¹, Zhengying Gu², Yannan Yang¹, Larry Cai¹, Shevanuja Theivendran¹, Yue Wang¹, Min Zhang², Chengzhong Yu^{1

2}

Affiliations

¹ Australian Institute for Bioengineering and Nanotechnology, The University of Queensland St Lucia Brisbane QLD 4072 Australia c.yu@uq.edu.au.
² School of Chemistry and Molecular Engineering, East China Normal University Shanghai 200241 China czyu@chem.ecnu.edu.cn.

Abstract

Pyroptosis is a programmed cell death widely studied in cancer cells for tumour inhibition, but rarely in dendritic cell (DC) activation for vaccine development. Here, we report the synthesis of sodium stabilized mesoporous aluminosilicate nanoparticles as DC pyroptosis modulators and antigen carriers. By surface modification of sodium-stabilized four-coordinate aluminium species on dendritic mesoporous silica nanoparticles, the resultant Na-^IVAl-DMSN significantly activated DC through caspase-1 dependent pyroptosis via pH responsive intracellular ion exchange. The released proinflammatory cellular contents further mediated DC hyperactivation with prolonged cytokine release. In vivo studies showed that Na-^IVAl-DMSN induced enhanced cellular immunity mediated by natural killer (NK) cells, cytotoxic T cells, and memory T cells as well as humoral immune response. Our results provide a new principle for the design of next-generation nanoadjuvants for vaccine applications.