Interference-resistant aptasensor with tetrahedral DNA nanostructure for profenofos detection based on the composites of graphene oxide and polyaniline

Guanjie Wang; Haowei Dong; Jie Han; Mei Zhang; Jingcheng Huang; Jiashuai Sun; Fukai Guan; Zheng Shen; Deyan Xu; Xia Sun; Yemin Guo; Shancang Zhao

doi:10.1016/j.bioelechem.2022.108227

Interference-resistant aptasensor with tetrahedral DNA nanostructure for profenofos detection based on the composites of graphene oxide and polyaniline

Bioelectrochemistry. 2022 Dec:148:108227. doi: 10.1016/j.bioelechem.2022.108227. Epub 2022 Aug 11.

Authors

Guanjie Wang¹, Haowei Dong¹, Jie Han¹, Mei Zhang¹, Jingcheng Huang¹, Jiashuai Sun¹, Fukai Guan¹, Zheng Shen¹, Deyan Xu¹, Xia Sun¹, Yemin Guo², Shancang Zhao³

Affiliations

¹ School of Agricultural Engineering and Food Science, Shandong University of Technology, No.266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No.266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No.266 Xincun Xilu, Zibo, Shandong 255049, China.
² School of Agricultural Engineering and Food Science, Shandong University of Technology, No.266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No.266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No.266 Xincun Xilu, Zibo, Shandong 255049, China. Electronic address: gym@sdut.edu.cn.
³ School of Agricultural Engineering and Food Science, Shandong University of Technology, No.266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No.266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No.266 Xincun Xilu, Zibo, Shandong 255049, China; Institute of Quality Standard and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China. Electronic address: shancangzhao@126.com.

PMID: 35973324
DOI: 10.1016/j.bioelechem.2022.108227

Abstract

In this work, an interference-resistant electrochemical aptasensor that could detect profenofos in vegetables was constructed based on complexes of graphene oxide and polyaniline (GO@PANI) and gold nanoparticles-tetrahedral DNA nanostructure (Au-TDN). Compared with a single chain aptamer, the tetrahedral DNA nanostructure is highly stable and allows the aptamer on this structure to stand in a highly ordered position on an electrode surface. Moreover, the AuNPs are biocompatible and can protect the activity of the aptamer, which can improve the assembly success rate of Au-TDN. Besides, the conductivity of PANI had been tremendously enhanced thanks to the existence of GO, which improved the dispersion of PANI. The GO@PANI was prepared by a chemical synthesis method, which had a large surface area and was able to adsorb many Au-TDN. Under optimal working parameters, the constructed aptasensor exhibited good electrochemical sensing performance with a detection limit of 10.50 pg/mL and a linear range of 1.0 × 10²-1.0 × 10⁷ pg/mL. In addition, it was employed in detecting profenofos in vegetables with a good recovery rate of 90.41-116.37 %. More importantly, the aptasensor also has excellent stability and high selectivity. This study provides a promising method to avoid interference in the detection of profenofos by sensors.

Keywords: Electrochemical aptasensor; Gold nanoparticles-tetrahedral DNA nanostructure; Graphene oxide and polyaniline; Profenofos.

MeSH terms

Aniline Compounds
Biosensing Techniques* / methods
DNA / chemistry
Electrochemical Techniques / methods
Gold / chemistry
Graphite
Metal Nanoparticles* / chemistry
Nanostructures*
Organothiophosphates

Substances

Aniline Compounds
Organothiophosphates
graphene oxide
polyaniline
Gold
Graphite
profenofos
DNA