Sigesbeckia orientalis Extract Ameliorates the Experimental Diabetic Nephropathy by Downregulating the Inflammatory and Oxidative Stress Signaling Pathways

Chung-Ming Chen; Jer-Yiing Houng; Tsui-Ling Ko; Shu-Hui Juan; Hsiu-Chu Chou

doi:10.1155/2022/3323745

Sigesbeckia orientalis Extract Ameliorates the Experimental Diabetic Nephropathy by Downregulating the Inflammatory and Oxidative Stress Signaling Pathways

Evid Based Complement Alternat Med. 2022 Aug 5:2022:3323745. doi: 10.1155/2022/3323745. eCollection 2022.

Authors

Chung-Ming Chen^{1

2}, Jer-Yiing Houng^{3

4}, Tsui-Ling Ko⁵, Shu-Hui Juan^{6

7}, Hsiu-Chu Chou^{6

8}

Affiliations

¹ Department of Pediatrics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.
² Department of Pediatrics, Taipei Medical University Hospital, Taipei 11031, Taiwan.
³ Graduate Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan.
⁴ Department of Nutrition, I-Shou University, Kaohsiung 82445, Taiwan.
⁵ College of Science, National Sun Yat-Sen University, Kaohsiung 82445, Taiwan.
⁶ Graduate Institute of Medical Sciences and Taipei Medical University, Taipei 11031, Taiwan.
⁷ Department of Physiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.
⁸ Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.

Abstract

Diabetes in children and its complications are on the rise globally, which is accompanied by increasing in diabetes-related complications. Oxidative stress and inflammation induced by elevated blood sugar in diabetic patients are considered risk factors associated with the development of diabetes complications, including chronic kidney disease and its later development to end-stage renal disease. Microvascular changes within the kidneys of DM patients often lead to chronic kidney disease, which aggravates the illness. Sigesbeckia orientalis extract (SOE), reported to have strong antioxidative and excellent anti-inflammatory activities, is used in the modern practice of traditional Chinese medicine. Kidneys from three groups of control mice (CTR), mice with streptozotocin (STZ)-induced diabetes (DM), and mice with STZ-induced DM treated with SOE (DMRx) were excised for morphological analyses and immunohistochemical assessments. Only mice in the DM group exhibited significantly lower body weight, but higher blood sugar was present. The results revealed more obvious renal injury in the DM group than in the other groups, which appeared as greater glomerular damage and tubular injury, sores, and plenty of connective tissues within the mesangium. Not only did the DM group have a higher level of cytokine, tumor necrosis factor, and the oxidative stress marker, 8-hydroxyguanosine expression, but also factors of the nuclear factor pathway and biomarkers of microvascular status had changed. Disturbances to the kidneys in DMRx mice were attenuated compared to the DM group. We concluded that SOE is an effective medicine, with antioxidative and anti-inflammatory abilities, to protect against or attenuate diabetic nephropathy from inflammatory disturbances by oxidative stress and to cure vessel damage in a hyperglycemic situation.