Assembly and phylogenetic analysis of the mitogenome of Piophila megastigmata (Diptera: Piophilidae), a forensically important species

Mitochondrial DNA B Resour. 2022 Aug 8;7(8):1451-1453. doi: 10.1080/23802359.2022.2107451. eCollection 2022.

Abstract

Piophila megastigmata (McAlpine, 1978) (Diptera: Piophilidae) is a forensically important species, but it was not found in China until recently. In this study, the first complete mitogenome of P. megastigmata was sequenced using the next-generation sequencing, which was also the first mitogenome of Piophilidae. Our mitochondrial assembly has a length of 15,410 bp, which consists of 22 tRNA genes, 13 protein-coding genes (PCGs), two rRNA genes, and a non-coding control region. This study provides a molecular basis to infer the minimum postmortem interval with P. megastigmata and a better understanding of the phylogeny of the order Diptera.

Keywords: Mitogenome; Piophilidae; evolution; necrophagous fly.

Grants and funding

This work was supported by the National Natural Science Foundation of China [grant number 31872258, 32070508, and 82002007].