Targeted next-generation sequencing for comprehensive genetic analysis of external apical root resorption during orthodontic treatment with premolar extraction in the Korean population

Yun-Ju Lee; Hayeon Pak; Chung-Ju Hwang; Yoon Jeong Choi; Jae-Hoon Lee; Ji Hyun Lee; Jung-Yul Cha

doi:10.1016/j.ajodo.2021.06.022

Targeted next-generation sequencing for comprehensive genetic analysis of external apical root resorption during orthodontic treatment with premolar extraction in the Korean population

Am J Orthod Dentofacial Orthop. 2022 Nov;162(5):668-679.e5. doi: 10.1016/j.ajodo.2021.06.022. Epub 2022 Aug 11.

Authors

Yun-Ju Lee¹, Hayeon Pak², Chung-Ju Hwang¹, Yoon Jeong Choi¹, Jae-Hoon Lee³, Ji Hyun Lee⁴, Jung-Yul Cha⁵

Affiliations

¹ Department of Orthodontics, Yonsei University College of Dentistry, Seodaemoon-gu, Seoul, South Korea.
² Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Dongdaemoon-gu, Seoul, South Korea.
³ Department of Prosthodontics, Yonsei University College of Dentistry, Seodaemoon-gu, Seoul, South Korea.
⁴ Department of Clinical Pharmacology and Therapeutics, College of Medicine, and Department of Biomedical Science and Technology, Kyung Hee Medical Science Research Institute, and Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Dongdaemun-gu, Seoul, South Korea. Electronic address: hyunihyuni@khu.ac.kr.
⁵ Department of Orthodontics, Yonsei University College of Dentistry, Seodaemoon-gu, Seoul, South Korea. Electronic address: jungcha@yuhs.ac.

PMID: 35965166
DOI: 10.1016/j.ajodo.2021.06.022

Abstract

Introduction: External apical root resorption (EARR) is one of the most common unfavorable consequences of orthodontic treatment and causes loss of tooth structure. The present study aimed to investigate the genetics of EARR using next-generation sequencing comprehensively.

Methods: Targeted next-generation sequencing was performed for comprehensive genetic analysis of 118 Korean orthodontic patients. The patients were divided into 2 groups on the basis of their EARR value. The association of clinical and genetic parameters with EARR was assessed using the χ² test or t test for matched pairs, followed by Bonferroni correction and linear regression analysis. In addition, haplotype analysis and in silico prediction were conducted to evaluate functional effects.

Results: No statistically significant difference was observed between clinical and treatment-related parameters and EARR. The single nucleotide polymorphisms SPP1 rs9138 (P = 0.001) and SFRP2 rs3810765 (P = 0.04) showed only nominal significance between EARR groups. However, these 2 SNPs were not significant after Bonferroni correction for multiple testing (cutoff P = 0.05/142 = 3.52 × 10^-4). Variations in SPP1 rs9138 and SFRP2 rs3810765 may be related to EARR during orthodontic treatment. In summary, not only genes related to inflammatory reactions but also those related to Wnt signaling to affect the degree of EARR during orthodontic teeth movement.